PUBLICATIONS



 

Huber, P. W. and K. G. Brandt (1980) "Kinetic Studies of the Mechanism of Pyridine Nucleotide Dependent Reduction of Yeast Glutathione Reductase" Biochemistry 19, 4568-4575.

 

Ulbrich, N., Y. L., Chan, P. W. Huber, and I. G. Wool (1982) "Separate Binding Sites on Rat Liver Ribosomal Protein L6 for 5S and 5.8S Ribosomal Ribonucleic Acids and for Transfer Ribonucleic Acids" J. Biol. Chem. 257, 11353-11357.

 

Endo, Y., P. W. Huber, and I. G. Wool (1983) "The Ribonuclease Activity of the Cytotoxin a-Sarcin: The characteristics of the enzymatic activity of a-sarcin with ribosomes and ribonucleic acids as substrates" J. Biol. Chem. 258, 2662-2667.

 

Huber, P. W. and I. G. Wool (1984) "Nuclease Protection Analysis of Ribonucleoprotein Complexes: Use of the cytotoxic ribonuclease a-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA" Proc. Natl. Acad. Sci. (USA) 81, 322-326.

 

Huber, P. W. and K. G. Brandt (1985) "Kinetic Studies of the Reduction of Yeast Glutathione Reductase by Reduced Nicotinamide Hypoxanthine Dinucleotide Phosphate" Arch. Biochem. Biophys. 238, 213-218.

 

Huber, P. W. and I. G. Wool (1986) "Use of the Cytotoxic Nuclease a-Sarcin to Identify the Binding Site on Eukaryotic 5S Ribosomal Ribonucleic Acid for the Ribosomal Protein L5"  J. Biol. Chem. 261, 3002-3005.

 

Huber, P. W. and I. G. Wool (1986) "Identification of the Binding Site on 5S rRNA for the Transcription Factor IIIA: Proposed structure of a common binding site on 5S rRNA and on the gene" Proc. Natl. Acad. Sci. (USA) 83, 1593-1597.

 

Huber, P. W. and I. G. Wool (1988) "The Use of a-Sarcin to Probe 5S rRNA-protein Interactions" Methods in Enzymology (Moldave, K., and Noller, H. F., eds), Academic Press, Inc. 164, 468-475.

 

Huber, P. W., G. C. Blobe and K. M. Hartmann (1991) "Conformational Studies of the Nucleic Acid Binding Sites for Xenopus Transcription Factor IIIA" J. Biol. Chem. 266, 3278-3286.

 

Darsillo, P. and P. W. Huber (1991) "The Use of Chemical Nucleases to Analyze RNA-Protein Interactions: The TFIIIA•5S rRNA Complex: J. Biol. Chem. 226, 21075-21082.

 

Huber, P. W., T. Morii, H.-Y. Mei and J. K. Barton (1991) "Structural Polymorphism in the Major Groove of a 5S RNA Gene Complements the Zinc Finger Domains of Transcription Factor IIIA" Proc. Natl. Acad. Sci. USA, 88, 10801-10805.

 

Chow, C. S., K. M. Hartmann, S. L. Rawlings, P. W. Huber and J. K. Barton (1992) "Delineation of Structural Domains in Eukaryotic 5S rRNA with a Rhodium Probe, Biochemistry 31, 3534-3542.

 

Huber, P. W. (1993) "Chemical Nucleases: Their Use in Studying RNA Structure and RNA-Protein Interactions" FASEB J., 7, 1367-1375.

 

Ghosh, M., L. J. Lambert, P. W. Huber and M. J. Miller (1995) "Synthesis, Bioactivity and DNA-Cleaving Ability of Desferrioxamine ß-Nalidixic Acid and Anthraquinone Carboxylic Acid Conjugates" Bioorg. Med. Chem. Lett., 5, 2337-2340.

 

Scripture, J. B., and P. W. Huber (1995) "Analysis of the Binding of Xenopus Ribosomal Protein L5 to Oocyte 5S rRNA: The Major Determinants of Recognition are Located in Helix III-Loop C" J. Biol. Chem., 270, 27358-27365.

 

Rawlings, S. L., G. D. Matt and P. W. Huber (1996) "Analysis of the Binding of Xenopus Transcription Factor IIIA to Ooctye 5S rRNA and to the 5S rRNA Gene" J. Biol. Chem. 271, 869-877.

 

Wang, S., P. W. Huber, M. Cui, A. W. Czarnik, and H.-Y. Mei (1998) "Binding of Neomycin to the TAR Element of HIV-1 RNA Induces Dissociation of Tat Protein by an Allosteric Mechanism" Biochemistry 37, 5549-5557.

 

Westmark, C. J., R. Ghose, and P. W. Huber (1998) "Inhibition of RNA Polymerase III Transcription by a Ribosome-Associated Kinase Activity" Nucleic Acids Res. 26, 4758-4764.

 

Zhao, W.-M., C. Jiang, T. T. Kroll, and P. W. Huber (2001) "A Proline-rich Protein Binds to the Localization Element of Xenopus Vg1 mRNA and to Ligands Involved in Actin Polymerization" EMBO J. 20, 2315-2325.

 

Huber, P. W., J. P. Rife, and P. B. Moore (2001) "The Structure of Helix III in Xenopus Oocyte 5S rRNA:  An RNA Stem Containing a Two-Nucleotide Bulge" J. Mol. Biol. 312, 823-832.

 

DiNitto, J. P. and P. W. Huber (2001) "A Role for Aromatic Amino Acids in the Binding of Xenopus Ribosomal Protein L5 to 5S rRNA" Biochemistry 40, 12645-12653.

 

Westmark, C. J., R. Ghose, and P. W. Huber (2002) "Phosphorylation of Xenopus Transcription Factor IIIA by an Oocyte Protein Kinase CK2" Biochem. J. 362, 375-382.

 

Kroll, T. T., W.-M. Zhao, C. Jiang, and P. W. Huber (2002) "A Homolog of FBP2/KSRP Binds to Localized mRNAs in Xenopus Oocytes" Development 129, 5609-5619.

 

Kolev, N. G. and P. W. Huber (2003) "VgRBP71 Stimulates Cleavage at a Polyadenylation Signal in Vg1 mRNA, Resulting in the Removal of a Cis-acting Element that Represses Translation" Mol. Cell 11, 745-755.

 

DiNitto, J. P. and P. W. Huber (2003) "Mutual Induced Fit Binding of Xenopus Ribosomal Protein L5 to 5S rRNA" J. Mol. Biol.   330, 979-992.

 

Ghose, R., M. Q. Malik, and P. W. Huber (2004) "Restricted Specificity of Xenopus TFIIIA for Transcription of Somatic-Type 5S rRNA Genes" Mol. Cell. Biol.  24, 2467-2477.

 

Sarveswaran, K., W. Hu, P. W. Huber, G. H. Bernstein, and M. Lieberman (2006) “Deposition of DNA Rafts on Cationic SAMs on Silicon [100]” Langmuir 22, 11279-11283.

 

Gellett, A. M., P. W. Huber, and P. J. Higgins (2008) “Synthesis of the Unnatural Amino Acid Na-Ne-(Ferrocene-1-acetyl)-L-lysine: A Novel Organometallic Nuclease”, J. Organomet. Chem.  693, 2959-2962.

 

Kolev, N. G., E. I. Hartland, and P. W. Huber (2008) “A Manganese-Dependent Ribozyme in the 3’-Untranslated Region of Xenopus Vg1 mRNA”  Nucleic Acids Res. 36, 5530-5539.

 

Kroll, T. T., L. B. McLaughlin, E. I. Hartland, D. D. Snedden, H. V. Goodson, and P. W. Huber (2009)  “Interactions of 40LoVe within the Ribonucleoprotein Complex that Forms on the Localization Element of Xenopus Vg1 mRNA” Mech. Dev. 126, 523-538. (Cover)

 

Huber, P. W. and W.-m. Zhao (2010)  “Detection of Protein-RNA Complexes in Xenopus Oocytes” Methods  51, 82-86.

 

Scripture, J. B. and P. W. Huber (2011) "Binding Site for Xenopus Ribosomal Protein L5 and Accompanying Structural Changes in 5S rRNA" Biochemistry 50, 3827-3839.

 

Snedden, D. D., M. M. Bertke, D. Vernon, and P. W. Huber (2013) "RNA Localization in Xenopus Oocytes Uses a Core Group of trans-acting Factors Irrespective of Destination" RNA 19, 889-895.

 

Sun, L., M. M. Bertke, M. M. Champion, G. Zhu, P. W. Huber, and N. J. Dovichi (2014)  “Quantitative Proteomics of Xenopus laevis Embryos: Expression kinetics of nearly 4000 proteins during early development” Sci. Reports, 4, 4365; DOI:10.1038/srep04365

 

Malik, M. Q., M. M. Bertke, and P. W. Huber (2014) “Small Ubiquitin-like Modifier (SUMO)-Mediated Repression of the Xenopus Oocyte 5S rRNA Genes” J. Biol. Chem.  289, 35468-35481.

 

Lambert, L. J., M. J. Miller, and P. W. Huber (2015) “Tetrahydrofuranyl and Tetrahydropyranyl Protection of Amino Acid Side-Chains Enables Synthesis of a Hydroxamate-Containing Aminoacylated tRNA” Org. Biomol. Chem. 13, 2341-2349.

 

Sun, L., M. M. Champion, P. W. Huber, and N. J. Dovichi (2016) “Proteomics of Xenopus Development”  Mol. Hum. Reprod. 22, 193-199.

 

Zhang, Z., L. Sun, G. Zhu, O. F. Cox, P. W. Huber, and N. J. Dovichi (2016) “Nearly 1000 Protein Identifications from 50 ng of Xenopus laevis Zygote Homogenate Using Online Sample Preparation on a Strong Cation Exchange Monolith Based Microreactor Coupled with Capillary Zone Electrophoresis”  Anal. Chem. 88, 877-882.

 

Sun, L., K. M. Dubiak, E. H. Peuchen, Z. Zhang, G. Zhu, P. W. Huber, and N. J. Dovichi (2016) “Single Cell Proteomics Using Frog (Xenopus laevis) Blastomeres Isolated from Early Stage Embryos, Which Form a Geometric Progression in Protein Content” Anal. Chem. 88, 6653-6657.

 

Peuchen, E. H., O. F. Cox, L. Sun, A. Hebert, J. J. Coon, M. F. Champion, N. J. Dovichi, and P. W. Huber (2017) “Phosphorylation Dynamics Dominate the Regulated Proteome

during Early Xenopus Development” Sci. Reports  7, 15647; DOI: 10.1038/s41598-017-15936-y

 

Jarvis, T. S., F. M. Roland, K. M. Dubiak, P. W. Huber, and B. D. Smith (2018) “Time-lapse imaging of cell death in cell culture and whole living organisms using turn-on deep-red fluorescent probes” J. Mater. Chem. B, 6, 4963-4971.

 

Cox, O. F., and P. W. Huber (2019) “Developing Practical Theraputic Strategies that Target Protein SUMOylation” Curr. Drug Targets 20, 960-969.

 

Bertke, M. M., K. M. Dubiak, L. Cronin, E. Zeng, and P. W. Huber (2019) “A Deficiency in SUMOylation Activity Disrupts Multiple Pathways Leading to Neural Tube and Heart Defects in Xenopus Embryos” BMC Genomics 20:386; DOI: 10.1186/s12864-019-5773-3.

 

Wang, M., K. M. Dubiak, Z. Zhang, P. W. Huber, D. D. Y. Chen, and N. J. Dovichi (2019) “MALDI-imaging of Early Stage Xenopus laevis Embryos” Talanta 204, 138-144.

 

Qu, Y., K. M. Dubiak, E. H. Peuchen, M. M. Champion, Z. Zhang, A. S. Hebert, S. Wright, J. J. Coon, P. W. Huber, and N. J. Dovichi (2020) "Quantitative Capillary Zone Electrophoresis-Mass Spectrometry Reveals the N-Glycome Developmental Plan during Vertebrate Embryogenesis" Mol. Omics 16, 210-220.

 

Zhang, Z., K. M. Dubiak, P. W. Huber, and N. J. Dovichi (2020) "Miniaturized Filter-Aided Sample Preparation (MICRO-FASP) Method for High Throughput, Ultrasensitive Proteomics Sample Preparation Reveals Proteome Asymmetry in Xenopus laevis Embryos" Anal. Chem. 92, 5554–5560.

 

Zhang, Z., K. M. Dubiak, E. Shishkova, P. W. Huber, J. J. Coon,and N. J. Dovichi (2022) “High-Throughput, Comprehensive Single-Cell Proteomic Analysis of Xenopus laevis Embryos at the 50-Cell Stage Using a Microplate-Based MICROFASP System”  Anal. Chem. 94, 3254-3259.

 

Yang, C., G. M. Dominique, M. M. Champion, P. W. Huber (2022) “Remnants of the Balbiani body are required for the formation of RNA transport Granules in Xenopus Oocytes”  iScience 25, 103878.